Composite

Part:BBa_K209455:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-hM4D-Beta Pix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2726
    Illegal XbaI site found at 4148
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2881
    Illegal PstI site found at 3147
    Illegal PstI site found at 3468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2081
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2881
    Illegal PstI site found at 3147
    Illegal PstI site found at 3468
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal XhoI site found at 3030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2726
    Illegal XbaI site found at 4148
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2881
    Illegal PstI site found at 3147
    Illegal PstI site found at 3468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2726
    Illegal XbaI site found at 4148
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2881
    Illegal PstI site found at 3147
    Illegal PstI site found at 3468
    Illegal NgoMIV site found at 1952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 2931
    Illegal BsaI.rc site found at 4233
    Illegal SapI.rc site found at 2537


Design Notes

Cloned into pEF5/FRT/V5-DEST.


Source

References